YT medium
Andreas Sagen
Published: 2023-03-20 DOI: 10.17504/protocols.io.kxygx9qx4g8j/v1
Abstract
2xYT is a nutritionally rich liquid microbial growth medium used for propagation of recombinant strains of Escherichia coli and M13 bacteriophage or other filamentous single-stranded DNA bacteriophages.
Steps
500 mL 2x YT medium
1.
Fill the bottle with 400mL deionized water
2.
Measure and add:
`8g` Tryptone
`5g` Yeast extract
`2.5g` Sodium chloride
Powdered compounds:
3.
Adjust pH to 7.0 by adding drops of concentrated NaOH
4.
Add deionized water to a total of 500mL
5. Cool to 50°C and supplement with antibiotics as appropriate
Autoclave liquid at 121°C for 0h 15m 0s
Note
