An analytical workflow for dynamic characterization and quantification of metal-bearing nanomaterials in biological matrices

Iseult Lynch, Peng Zhang, Fazel Abdolahpur Monikh, Zhiling Guo, Martina G. Vijver, Eugenia Valsami-Jones, Willie J. G. M. Peijnenburg

Published: 2022-06-28 DOI: 10.1038/s41596-022-00701-x

Quantification

Metal-bearing nanomaterials

Biological matrices

Dynamic characterization

Analytical workflow

AI 解读

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To assess the safety of engineered nanomaterials (ENMs) and to evaluate and improve ENMs’ targeting ability for medical application, it is necessary to analyze the fate of these materials in biological media. This protocol presents a workflow that
Introduction
Procedure 1: insitu quantification of cellular uptake of M-nobreakENMs by scICP-MS analysis
- Sample preparation
- Quantification of cellular association using scICP-MS
Procedure 2: characterization and quantification of M-nobreakENMs from cells, microorganisms and tissues of organisms following particle extraction
- Extraction of M-nobreakENMs from (micro)organisms, cells and tissues
- Characterization of the extracted M-nobreakENM
Procedure 3: insitu characterization of M-nobreakENMs biotransformation in biological matrices using synchrotron radiation-based techniques
- Procedure
Troubleshooting
Timing
Procedure 1
Procedure 2
- Procedure
Procedure 3
Anticipated results
- References
References
ACKNOWLEDGMENTS

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An analytical workflow for dynamic characterization and quantification of metal-bearing nanomaterials in biological matrices

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