Subcellular patch-clamp techniques for single-bouton stimulation and simultaneous pre- and postsynaptic recording at cortical synapses

David Vandael, Yuji Okamoto, Carolina Borges-Merjane, Victor Vargas-Barroso, Benjamin A. Suter, Peter Jonas

Published: 2021-05-13 DOI: 10.1038/s41596-021-00526-0

patch-clamp

cortical synapses

presynaptic recording

postsynaptic recording

unitary synaptic events

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Rigorous investigation of synaptic transmission requires analysis of unitary synaptic events by simultaneous recording from presynaptic terminals and postsynaptic target neurons. However, this has been achieved at only a limited number of model synapses,
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
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ACKNOWLEDGMENTS

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Subcellular patch-clamp techniques for single-bouton stimulation and simultaneous pre- and postsynaptic recording at cortical synapses

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