Single-cell RNA-seq of maize meiocytes and pollen grains

Mary Washburn, Jaime Alaniz-Fabián, Taylor Scroggs, Brad Nelms

Published: 2023-10-01 DOI: 10.1038/s41596-023-00889-6

single-cell RNA-seq

maize meiocytes

pollen grains

cell heterogeneity

CEL-Seq2

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Supplementary information

Supplementary Information

Supplementary Figs. 1 and 2 and Supplementary Table 2

Supplementary Table

Supplementary Table 1. Sequence of barcoded CEL-seq primers. The first of these primers is shown in Supplementary Figure 2a with each section annotated. This table can be directly uploaded to IDT for ordering plate-based oligos. Because there are randomized bases in these primers (Ns), the automated form will ask if you want ‘machine-mixed’ or ‘hand-mixed’ bases during ordering; select machine-mixed bases.

Supplementary Data 1

Supplementary Data 1. CELseq_whitelist_legacy.txt. Legacy CELseq whitelist for Salmon Alevin. Use when re-analyzing data from ref. 3.

Supplementary Data 2

Supplementary Data 2. TGMAP.tsv. Transcript to gene mapping for Salmon Alevin.

Supplementary Data 3

Supplementary Data 3. CELseq_whitelist.txt. CELseq whitelist for Salmon Alevin. Use when analyzing new data generated with this protocol.

Supplementary Video 1

Supplementary Video 1. Technique to press anthers and release meiotic cells or pollen precursors (Step 5A(v–vii) and 5B(ii)).

Supplementary Video 2

Supplementary Video 2. Technique to isolate single cells and pollen precursors by using a syringe needle (Steps 6–12).

Supplementary Video 3

Supplementary Video 3. Handling of single-cell material on PCR caps (Steps 14 and 22–25).

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References

Supplementary information

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关键词

single-cell RNA-seq

maize meiocytes

pollen grains

cell heterogeneity

CEL-Seq2

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