Sanger Tree of Life Wet Laboratory Protocol Collection
Caroline Howard, Amy Denton, Halyna Yatsenko, Jessie Jay, Karen E Houliston
Published: 2023-10-02 DOI: 10.17504/protocols.io.8epv5xxy6g1b/v1
reference genome
long read sequencing
sample preparation
tissue dissection
sample homogenisation
PowerMasher
cryoPREP
tissue disruption
cryogenic tissue disruption
plant tissue disruption
tissue homogenisation
cryogenic bead beating
HMW DNA extraction
magnetic bead extraction
automated DNA extraction
KingFisher
MagAttract
manual DNA extraction
plant HMW DNA extraction
solid phase reversible immobilisation
Nanobind
nucleated blood extractions
mollusc extraction
organic extraction
next generation sequencing
third generation sequencing
HMW DNA fragmentation
Megaruptor
low input
PacBio
ultra-low input
Covaris
g-TUBE
SPRI
automated SPRI
manual SPRI
AMPure PB beads
DNA clean-up
RNA extraction
manual RNA extraction
TRIzol
RNA-Seq
Iso-Seq
automated RNA extraction
MagMax
mirVana
magnetic beads
HMW DNA pooling
Abstract
This is the collection of Sanger Tree of Life Wet Laboratory protocols used for the generation of reference level genome assemblies.
This collection currently contains protocols which cover all stages of the wet laboratory processes performed by the Sanger Tree of Life; these processes are sample preparation, sample homogenisation, HMW DNA extraction, HMW DNA fragmentation, fragmented DNA clean-up and RNA extraction.
This collection will be updated as protocols within the Tree of Life Core Laboratory continue to be developed and refined.
