Researcher led sample preparation for LC-MS using the BioMS research core facility
ronan o'cualain
Published: 2022-09-09 DOI: 10.17504/protocols.io.261genmkdg47/v1
S-Trap™ column
Sample lysis
Speed vac
sample preparation
lc-ms
proteomics
biochemistry
reduction and alkylation
ultrasonication
micro-proteomics
plate-based
throughput
S-trap plate
protifi
covaris
millipore
direct detect
protein
peptides
mass spectrometry
desalting
cleanup
clean-up
processing
enzymatic digestion
trypsin
R3
reverse-phase
Abstract
This is a collection of protocols that covers the processing of most biological samples for proteomics, from collection of sample, up to data acquisition using LC-MS
Before start
This method is suitable for the preparation of a wide variety of sample types for LC-MS analysis. It is assumed that you have already had a discussion about your work before attempting the steps described here. If not, contact us at bioms@manchester.ac.uk .
To get you started, we will provide you with Covaris lysis tubes for each sample that you wish to process, at cost.
