Quick-Start Protocol for DNeasy® PowerSoil® Pro Kit

Spin the PowerBead Pro Tube briefly to ensure that the beads have settled at the bottom. Add up to 250mg of soil and 800µL of Solution CD1. Vortex briefly to mix.

Secure the PowerBead Pro Tube horizontally on a Vortex Adapter for 1.5–2 ml tubes (cat. no. 13000-V1-24). Vortex at maximum speed for 0h 10m 0s

Centrifuge the PowerBead Pro Tube at 15000x g,0h 0m 0s for 0h 1m 0s

Transfer the supernatant to a clean 2 ml Microcentrifuge Tube (provided)

Add 200µL of Solution CD2 and vortex for 0h 0m 5s

Centrifuge at 15000x g,0h 0m 0s for 0h 1m 0s at Room temperature . Avoiding the pellet, transfer up to 700µL of supernatant to a clean 2 ml Microcentrifuge Tube (provided)

Add 600µL of Solution CD3 and vortex for 0h 0m 5s .

Load 650µL of the lysate onto an MB Spin Column and centrifuge at 15000x g,0h 0m 0s for 0h 1m 0s

Discard the flow-through and repeat step 8 to ensure that all of the lysate has passed through the MB Spin Column.

0. Carefully place the MB Spin Column into a clean 2 ml Collection Tube (provided).

Add 500µL of Solution EA to the MB Spin Column. Centrifuge at 15000x g,0h 0m 0s for 0h 1m 0s

. Discard the flow-through and place the MB Spin Column back into the same 2 ml Collection Tube.

Add 500µL of Solution C5 to the MB Spin Column. Centrifuge at 15000x g,0h 0m 0s for 0h 1m 0s

Discard the flow-through and place the MB Spin Column into a new 2 ml Collection Tube (provided).

Centrifuge at up to 16000x g,0h 0m 0s for 0h 2m 0s . Carefully place the MB Spin Column into a new 1.5 ml Elution Tube (provided)

. Add 50mL -100mL of Solution C6 to the center of the white filter membrane.

Centrifuge at 15000x g,0h 0m 0s for 0h 1m 0s . Discard the MB Spin Column. The DNA is now ready for downstream applications