Preparation of 1M calcium chloride solution (CaCl2)
Alfonso Pérez Escudero, Alid Al-Asmar, gabrielmadirolas
Abstract
Original source of the protocol: WormBook Methods http://www.wormbook.org/chapters/www_strainmaintain/strainmaintain.html
This protocol is for making 1M calcium chloride solution, which is used in the preparation of worm media, like NGM (Nematode Growth Medium).
Steps
Weigh 7.4g of calcium chloride dihydrate (CaCl2·2H2O).
Dissolve it in ~40 mL of milliQ water in a 100mL measuring cylinder.
Add a clean stirring magnet, and leave it on a stirrer until all crystals are dissolved.
Remove the stirring magnet: To prevent touching the solution, use another magnet from the outside of the measuring cylinder to slide the stirring magnet out of the cylinder.
Fill up to 50mL.
Filter-sterilize.
Store at 4°C.
We usually use it without problem for up to at least a year after preparation.
