Plasma Exosome Isolation
Silvia Cerri
Abstract
This protocol details methods for the isolation of small extracellular vesicles from plasma.
Before start
Keep samples On ice during the entire procedure!
Steps
Plasma Exosome Isolation
Incubate plasma samples (starting volume: 1-1.5ml) On ice for complete thaw.
Filter samples through a 13mm sterile syringe filters with a 0.8 µm Supor (PES) membrane.
Centrifuge at 20000x g,4°C to remove large extracellular vesicles.
Collect the supernatant and filter it through 13mm sterile syringe filters with 0.2 mm Supor (PES) membrane.
Centrifuge at 100000x g,4°C to pellet small extracellular vesicles.
Remove the supernatant, resuspend the pellet in 1mL and repeat the previous step (repeated again in the next step for convenience).
Centrifuge at 100000x g,4°C to pellet small extracellular vesicles.
Process the pellet according to the type of analysis.
