Open Field Protocol
Michael Lee
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Abstract
This protocol details about Open Field procedure.
Attachments
Steps
Set up
20 x 20 x 10 inch chamber (LxWxH).
Opaque chambers or dividers between chambers.
Light intensity = 250 lux.
Have chambers on enclosed rack to diminish outside light.
Test
AnyMaze
Set two zones in the chamber. Outer ~3.5 inch perimeter of box is one zone and the center of the maze is the other zone.
Record locomotor activity and track how much time the animal spends in each zone for 0h 30m 0s.
Wipe down chambers with ethanol between animals.
Run males before females if running both genders on same day.
Can be used as a simple locomotion test by tracking the distance traveled and speed.
Can also be used as an anxiety test. Less anxious animals will spend more time in the center of the maze.
