Genetic manipulation and immortalized culture of ex vivo primary human germinal center B cells

Rebecca Caeser, Jie Gao, Miriam Di Re, Chun Gong, Daniel J. Hodson

Published: 2021-04-08 DOI: 10.1038/s41596-021-00506-4

ex vivo expansion

germinal center B cells

CRISPR-Cas9

lymphoma models

functional genomics

AI 解读

Genetic manipulation and immortalized culture of ex vivo primary human germinal center B cells

The cell-line-derived subcutaneous tumor model in preclinical cancer research

Subcellular patch-clamp techniques for single-bouton stimulation and simultaneous pre- and postsynaptic recording at cortical synapses

NovoSpaRc: flexible spatial reconstruction of single-cell gene expression with optimal transport

Genome-wide pooled CRISPR screening in neurospheres

High-throughput cultivation and identification of bacteria from the plant root microbiota

Viral crosslinking and solid-phase purification enables discovery of ribonucleoprotein complexes on incoming RNA virus genomes

Mitochondrial single-cell ATAC-seq for high-throughput multi-omic detection of mitochondrial genotypes and chromatin accessibility

Addendum: Micro-CT acquisition and image processing to track and characterize pulmonary nodules in mice

Multiplexed single-cell proteomics using SCoPE2

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Next-generation sequencing has transformed our knowledge of the genetics of lymphoid malignancies. However, limited experimental systems are available to model the functional effects of these genetic changes and their implications for therapy. The majorit
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Anticipated results
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ACKNOWLEDGMENTS

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Genetic manipulation and immortalized culture of ex vivo primary human germinal center B cells

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