ONT DNA Barcoding Fungal Amplicons w/ MinION & Flongle

Stephen Douglas Russell

Published: 2023-03-18 DOI: 10.17504/protocols.io.36wgq7qykvk5/v3

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Abstract

This collection of protocols outline a working methodology to DNA barcode fungal specimens. This process will work for dried tissue, fresh tissue, or with DNA template that has already gone through an alternative extraction protocol. It takes you from computer/software setup through DNA extraction, amplification, library preparation, and sequencing It also includes primary and secondary data analysis. Take your fungal samples from specimens to GenBank accessions with this protocol!

This particular workflow is designed for use with Flongle 10.4.1 flowcells and V14 Ligation chemistry.

Steps

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