Mouse Whole Cell Tissue Processing for 10x Genomics Platform
Allen Institute for Brain Science
Published: 2022-04-22 DOI: 10.17504/protocols.io.q26g7b52klwz/v9
10x
genomics
whole cell
transcriptomic
isolate
extraction
processing
preparation
cell sorting
cell suspension
PF0307
Abstract
This protocol describes how to isolate tissue from various ROI’s of the mouse brain followed by preparation of a highly concentrated single cell suspension for transcriptomic profiling on 10x Genomics platform.
Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
