Irreversible light-activated SpyLigation mediates split-protein assembly in 4D

Brizzia G. Munoz-Robles, Cole A. DeForest

Published: 2024-01-22 DOI: 10.1038/s41596-023-00938-0

LASL

SpyLigation

split-protein assembly

photocaging

protein activation

AI 解读

Genetic manipulation and immortalized culture of ex vivo primary human germinal center B cells

The cell-line-derived subcutaneous tumor model in preclinical cancer research

Subcellular patch-clamp techniques for single-bouton stimulation and simultaneous pre- and postsynaptic recording at cortical synapses

NovoSpaRc: flexible spatial reconstruction of single-cell gene expression with optimal transport

Genome-wide pooled CRISPR screening in neurospheres

High-throughput cultivation and identification of bacteria from the plant root microbiota

Viral crosslinking and solid-phase purification enables discovery of ribonucleoprotein complexes on incoming RNA virus genomes

Mitochondrial single-cell ATAC-seq for high-throughput multi-omic detection of mitochondrial genotypes and chromatin accessibility

Addendum: Micro-CT acquisition and image processing to track and characterize pulmonary nodules in mice

Multiplexed single-cell proteomics using SCoPE2

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Sections

Figures

References

Introduction
Materials
Procedure 1: Lys(oNB) synthesis
- Synthesis of 2-nitrobenzyl-N-succinimidyl carbonate
- Synthesis of Lys(oNB)
Procedure 2: split-protein design
Procedure 3: E. coli protein expression
Procedure 4: biomaterial incorporation
Procedure 5: mammalian cell culture and characterization
Troubleshooting
Timing
Anticipated results
Acknowledgements

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Irreversible light-activated SpyLigation mediates split-protein assembly in 4D

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