In vitro generation of self-renewing human intestinal epithelia over planar and shaped collagen hydrogels

Samuel S. Hinman, Yuli Wang, Raehyun Kim, Nancy L. Allbritton

Published: 2020-12-08 DOI: 10.1038/s41596-020-00419-8

intestinal epithelia

hydrogel scaffold

stem cells

crypt formation

growth factor gradients

AI 解读

Genetic manipulation and immortalized culture of ex vivo primary human germinal center B cells

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Subcellular patch-clamp techniques for single-bouton stimulation and simultaneous pre- and postsynaptic recording at cortical synapses

NovoSpaRc: flexible spatial reconstruction of single-cell gene expression with optimal transport

Genome-wide pooled CRISPR screening in neurospheres

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Viral crosslinking and solid-phase purification enables discovery of ribonucleoprotein complexes on incoming RNA virus genomes

Mitochondrial single-cell ATAC-seq for high-throughput multi-omic detection of mitochondrial genotypes and chromatin accessibility

Addendum: Micro-CT acquisition and image processing to track and characterize pulmonary nodules in mice

Multiplexed single-cell proteomics using SCoPE2

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The large intestine, with its array of crypts lining the epithelium and diverse luminal contents, regulates homeostasis throughout the body. In vitro crypts formed from primary human intestinal epithelial stem cells on a 3D shaped hydrogel scaffold replic
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
- Reporting Summary
- References
References
ACKNOWLEDGMENTS

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In vitro generation of self-renewing human intestinal epithelia over planar and shaped collagen hydrogels

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