Implementation of a 4Pi-SMS super-resolution microscope

Jingyu Wang, Edward S. Allgeyer, George Sirinakis, Yongdeng Zhang, Kevin Hu, Mark D. Lessard, Yiming Li, Robin Diekmann, Michael A. Phillips, Ian M. Dobbie, Jonas Ries, Martin J. Booth, Joerg Bewersdorf

Published: 2020-12-15 DOI: 10.1038/s41596-020-00428-7

4Pi-SMS microscopy

super-resolution

fluorescence microscopy

optomechanical assembly

troubleshooting guide

AI 解读

Supplementary information

Supplementary Information

Supplementary Methods and Supplementary Table 1.

Reporting Summary

Using high-resolution microscopy data to generate realistic structures for electromagnetic FDTD simulations from complex biological models

Experimental modulation of physiological force application on leg joint neurons in intactDrosophila melanogaster

Mitochondrial single-cell ATAC-seq for high-throughput multi-omic detection of mitochondrial genotypes and chromatin accessibility

Identification, isolation and analysis of human gut-associated lymphoid tissues

Development of RNA G-quadruplex (rG4)-targetingl-RNA aptamers by rG4-SELEX

Self-assembled ultraflexible probes for long-term neural recordings and neuromodulation

Hyperpolarized water as universal sensitivity booster in biomolecular NMR

Improved sampling and DNA extraction procedures for microbiome analysis in food-processing environments

An analytical workflow for dynamic characterization and quantification of metal-bearing nanomaterials in biological matrices

A flow cytometry-based protocol for syngenic isolation of neurovascular unit cells from mouse and human tissues

查看全部

Sections

Figures

References

Supplementary information

Shtengel, G. et al. Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure. Proc. Natl Acad. Sci. USA 106, 3125–3130 (2009).
Aquino, D. et al. Two-color nanoscopy of three-dimensional volumes by 4Pi detection of stochastically switched fluorophores. Nat. Methods 8, 353–359 (2011).
Wang, G., Hauver, J., Thomas, Z., Darst, S. A. & Pertsinidis, A. Single-molecule real-time 3D imaging of the transcription cycle by modulation interferometry. Cell 167, 1839–1852.e21 (2016).
Huang, F. et al. Ultra-high resolution 3D imaging of whole cells. Cell 166, 1028–1040 (2016).
Del Viso, F. et al. Congenital heart disease genetics uncovers context-dependent organization and function of nucleoporins at cilia. Dev. Cell 38, 478–492 (2016).
Zhang, Y., Lara-Tejero, M., Bewersdorf, J. & Galan, J. E. Visualization and characterization of individual type III protein secretion machines in live bacteria. Proc. Natl Acad. Sci. USA 114, 6098–6103 (2017).
Hwang, J. Y. et al. Dual sensing of physiologic pH and calcium by EFCAB9 regulates sperm motility. Cell 177, 1480–1494.e19 (2019).
Schroeder, L. K. et al. Dynamic nanoscale morphology of the ER surveyed by STED microscopy. J. Cell Biol 218, 83–96 (2019).
Zhang, Y. et al. Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging. Nat. Methods 17, 225–231 (2020).
Karanastasis, A. A. et al. 3D mapping of nanoscale crosslink heterogeneities in microgels. Mater. Horiz. 5, 1130–1136 (2018).
Brown, T. A. et al. Superresolution fluorescence imaging of mitochondrial nucleoids reveals their spatial range, limits, and membrane interaction. Mol. Cell. Biol. 31, 4994–5010 (2011).
Schermelleh, L. et al. Super-resolution microscopy demystified. Nat. Cell Biol. 21, 72–84 (2019).
Barentine, A. E. S. et al. 3D multicolor nanoscopy at 10,000 cells a day. Preprint at https://www.biorxiv.org/content/10.1101/606954v1 (2019).
Booth, M. J. Adaptive optical microscopy: the ongoing quest for a perfect image. Light Sci. Appl. 3, e165 (2014).
Burke, D., Patton, B., Huang, F., Bewersdorf, J. & Booth, M. J. Adaptive optics correction of specimen-induced aberrations in single-molecule switching microscopy. Optica 2, 177–185 (2015).
Van Engelenburg, S. B. et al. Distribution of ESCRT machinery at HIV assembly sites reveals virus scaffolding of ESCRT subunits. Science 343, 653–656 (2014).
Buttler, C. A. et al. Single molecule fate of HIV-1 envelope reveals late-stage viral lattice incorporation. Nat. Commun. 9, 1861 (2018).
Liu, S. & Huang, F. Enhanced 4Pi single-molecule localization microscopy with coherent pupil based localization. Commun. Biol. 3, 220 (2020).
Li, Y. et al. Accurate 4Pi single-molecule localization using an experimental PSF model. Opt. Lett. 45, 3765–3768 (2020).
Li, X. et al. Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM. Nat. Methods 10, 584–590 (2013).
Wang, Y. et al. Localization events-based sample drift correction for localization microscopy with redundant cross-correlation algorithm. Opt. Express 22, 15982–15991 (2014).
Li, Y. et al. Real-time 3D single-molecule localization using experimental point spread functions. Nat. Methods 15, 367–369 (2018).
Thevathasan, J. V. et al. Nuclear pores as versatile reference standards for quantitative superresolution microscopy. Nat. Methods 16, 1045–1053 (2019).
Antonello, J., Wang, J., He, C., Phillips, M. & Booth, M. Interferometric calibration of a deformable mirror. Zenodo. 19 March 2020 (accessed 21 November 2020) https://zenodo.org/record/3714951#.X6RM2lNKgdU

Implementation of a 4Pi-SMS super-resolution microscope

Supplementary information

推荐阅读