Immunostaining infiltrating spheroids as preparation for quantitative light-sheet imaging

Although various in vivo and in vitro models for studying glioblastoma cell invasion has progressed the field, there is still a need for optimized procedures. In particular to reveal key features of glioblastoma biology and infiltrating growth. In this protocol, we present an approach using indirect immunofluorescence in a 3D human xenograft glioblastoma spheroid model embedded in a naturally derived extracellular matrix

protocol.pdf