Functionalized tetrahedral DNA frameworks for the capture of circulating tumor cells

Yirong Chen, Meihua Lin, Dekai Ye, Shaopeng Wang, Xiaolei Zuo, Min Li

Published: 2024-02-05 DOI: 10.1038/s41596-023-00943-3

Circulating tumor cells

DNA frameworks

Aptamers

Targeted capture

Tetrahedral probes

AI 解读

Supplementary information

Supplementary Information

Supplementary Figs. 1–7

Reporting Summary

Supplementary Data 5

Unprocessed gels for Supplementary Fig. 5

Using high-resolution microscopy data to generate realistic structures for electromagnetic FDTD simulations from complex biological models

Experimental modulation of physiological force application on leg joint neurons in intactDrosophila melanogaster

Mitochondrial single-cell ATAC-seq for high-throughput multi-omic detection of mitochondrial genotypes and chromatin accessibility

Identification, isolation and analysis of human gut-associated lymphoid tissues

Development of RNA G-quadruplex (rG4)-targetingl-RNA aptamers by rG4-SELEX

Self-assembled ultraflexible probes for long-term neural recordings and neuromodulation

Hyperpolarized water as universal sensitivity booster in biomolecular NMR

Improved sampling and DNA extraction procedures for microbiome analysis in food-processing environments

An analytical workflow for dynamic characterization and quantification of metal-bearing nanomaterials in biological matrices

A flow cytometry-based protocol for syngenic isolation of neurovascular unit cells from mouse and human tissues

查看全部

Sections

Figures

References

Supplementary information

Alix-Panabières, C. & Pantel, K. Circulating tumor cells: liquid biopsy of cancer. Clin. Chem. 59, 110–118 (2013).
Ma, N. & Jeffrey, S. S. Deciphering cancer clues from blood. Science 367, 1424–1425 (2020).
Kelley, S. O. & Pantel, K. A new era in liquid biopsy: from genotype to phenotype. Clin. Chem. 66, 89–96 (2020).
Ozkumur, E. et al. Inertial focusing for tumor antigen–dependent and –independent sorting of rare circulating tumor cells. Sci. Transl. Med. 5, 179ra147 (2013).
Marquette, C. H. et al. Circulating tumour cells as a potential biomarker for lung cancer screening: a prospective cohort study. Lancet Respir. Med. 8, 709–716 (2020).
Steeg, P. S. Tumor metastasis: mechanistic insights and clinical challenges. Nat. Med. 12, 895–904 (2006).
Qin, W. et al. Bioinspired DNA nanointerface with anisotropic aptamers for accurate capture of circulating tumor cells. Adv. Sci. (Weinh.) 7, 2000647 (2020).
Song, Y. et al. Bioinspired engineering of a multivalent aptamer-functionalized nanointerface to enhance the capture and release of circulating tumor cells. Angew. Chem. Int. Ed. Engl. 58, 2236–2240 (2019).
Englund, E. A. et al. Programmable multivalent display of receptor ligands using peptide nucleic acid nanoscaffolds. Nat. Commun. 3, 614 (2012).
Zhao, W. et al. Bioinspired multivalent DNA network for capture and release of cells. Proc. Natl Acad. Sci. USA 109, 19626–19631 (2012).
Sheng, W., Chen, T., Tan, W. & Fan, Z. H. Multivalent DNA nanospheres for enhanced capture of cancer cells in microfluidic devices. ACS Nano 7, 7067–7076 (2013).
Yoon, H. J. et al. Sensitive capture of circulating tumour cells by functionalized graphene oxide nanosheets. Nat. Nanotechnol. 8, 735–741 (2013).
Shen, Z., Wu, A. & Chen, X. Current detection technologies for circulating tumor cells. Chem. Soc. Rev. 46, 2038–2056 (2017).
Gomes de Castro, M. A. et al. Differential organization of tonic and chronic B cell antigen receptors in the plasma membrane. Nat. Commun. 10, 820 (2019).
Huppa, J. B. et al. TCR–peptide–MHC interactions in situ show accelerated kinetics and increased affinity. Nature 463, 963–967 (2010).
Seeman, N. C. & Sleiman, H. F. DNA nanotechnology. Nat. Rev. Mater. 3, 17068 (2017).
Rothemund, P. W. K. Folding DNA to create nanoscale shapes and patterns. Nature 440, 297–302 (2006).
Li, M. et al. Nucleic acid tests for clinical translation. Chem. Rev. 121, 10469–10558 (2021).
Lin, M. et al. Programmable engineering of a biosensing interface with tetrahedral DNA nanostructures for ultrasensitive DNA detection. Angew. Chem. Int. Ed. Engl. 54, 2151–2155 (2015).
Liu, Q. et al. Valency-controlled framework nucleic acid signal amplifiers. Angew. Chem. Int. Ed. Engl. 57, 7131–7135 (2018).
Yin, F. et al. DNA-framework-based multidimensional molecular classifiers for cancer diagnosis. Nat. Nanotechnol. 18, 677–686 (2023).
Ye, D., Zuo, X. & Fan, C. DNA nanotechnology-enabled interfacial engineering for biosensor development. Annu. Rev. Anal. Chem. 11, 171–195 (2018).
Zhao, S. et al. A DNA origami-based aptamer nanoarray for potent and reversible anticoagulation in hemodialysis. Nat. Commun. 12, 358 (2021).
Wilner, O. I. & Willner, I. Functionalized DNA nanostructures. Chem. Rev. 112, 2528–2556 (2012).
Chandrasekaran, A. R., Anderson, N., Kizer, M., Halvorsen, K. & Wang, X. Beyond the fold: emerging biological applications of DNA origami. Chembiochem 17, 1081–1089 (2016).
Kacherovsky, N. et al. Traceless aptamer-mediated isolation of CD8+ T cells for chimeric antigen receptor T-cell therapy. Nat. Biomed. Eng. 3, 783–795 (2019).
Mao, M. et al. Modular DNA-origami-based nanoarrays enhance cell binding affinity through the “lock-and-key” interaction. J. Am. Chem. Soc. 145, 5447–5455 (2023).
Lin, M. et al. Electrochemical detection of nucleic acids, proteins, small molecules and cells using a DNA-nanostructure-based universal biosensing platform. Nat. Protoc. 11, 1244–1263 (2016).
Li, M. et al. DNA framework-programmed cell capture via topology-engineered receptor–ligand interactions. J. Am. Chem. Soc. 141, 18910–18915 (2019).
Zhang, J. L. et al. DNA nanolithography enables a highly ordered recognition interface in a microfluidic chip for the efficient capture and release of circulating tumor cells. Angew. Chem. Int. Ed. Engl. 59, 14115–14119 (2020).
Chen, J. et al. Biospecific monolayer coating for multivalent capture of circulating tumor cells with high sensitivity. Adv. Funct. Mater. 29, 1808961 (2019).
Mitchell, N. et al. A DNA nanostructure for the functional assembly of chemical groups with tunable stoichiometry and defined nanoscale geometry. Angew. Chem. Int. Ed. Engl. 48, 525–527 (2008).

Functionalized tetrahedral DNA frameworks for the capture of circulating tumor cells

Supplementary information

推荐阅读