Fresh Frozen / OCT Tissue RNA Quality Evaluation -- University of Minnesota TMCs (ThermoFisher Scientific, Invitrogen - Purelink)

Laura J Niedernhofer, David A Bernlohr, ThermoFisher Scientific

Published: 2023-08-10 DOI: 10.17504/protocols.io.dm6gpjpz8gzp/v1

RNA

QC

ThermoFisher

UMN

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Abstract

RNA quality evaluation of OCT (Optimal cutting temperature) or fresh frozen blocks/samples.

Completed prior to going through -omics and spatial technologies, sc/snRNAseq if applicable.

Steps

Purelink RNA Mini Kit Manual

1.

purelink_rna_mini_kit_man -- Reduced to pages of interest.pdf

2.

Product Information

Catalog Number: 12183018A

https://www.thermofisher.com/order/catalog/product/12183018A

or

Catalog Number: 12183020

https://www.thermofisher.com/order/catalog/product/12183020

3.

Complete:

Nanodrop for RNA quantification

RIN score for (Eukaryotic total) RNA qualification

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