Bedding Test

daniel.dautan daniel, Per Svenningsson

Published: 2024-05-23 DOI: 10.17504/protocols.io.n2bvj3ko5lk5/v1

Abstract

Used to assess nigrostriatal sensorimotor function in mice. Based on the Deacon 2006 protocol.

Steps

1.

Transfer the mice as single cage into a “Green line” cage (Techniplast, USA).

2.

Provide few pellet of food (Standard diet, SDS, R1/CRM/R3) (around 2-3 pellets for a total of 5g) as well as water ad libitum.

3.

Place a 5 x 5 cm cellulose paper tissue folded in the right corner of the cage.

4.

Place the cage in a ventilated rack with temperature, humidity and light control for 48h.

5.

At the end of the 48h check the animal cage and take a picture.

6.

Score the bedding as follow:

  1. The mice did not touch the paper square.

  2. The mice unfolded the paper square but did not tear it up.

  3. The mice unfolded and started to tore up <10% of the paper square.

  4. The mice tore up the paper square <50%

  5. The mice tore up the paper square, but the bedding is not perfect.

  6. Perfect bedding

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