BIOSPECIMENS SOPs Clemens Scherzer, Bradley Hyman, Charles Jennings Published: 2022-11-22 DOI: 10.17504/protocols.io.8epv5zerjv1b/v1 ASAPCRN AI 解读 浏览 1115 Abstract This collection explains Standard Operating Protocols for the Harvard Biomarkers Study. Steps 推荐阅读 Nature Protocols Protocols IO Current Protocols Genetic manipulation and immortalized culture of ex vivo primary human germinal center B cells The cell-line-derived subcutaneous tumor model in preclinical cancer research Subcellular patch-clamp techniques for single-bouton stimulation and simultaneous pre- and postsynaptic recording at cortical synapses NovoSpaRc: flexible spatial reconstruction of single-cell gene expression with optimal transport Genome-wide pooled CRISPR screening in neurospheres High-throughput cultivation and identification of bacteria from the plant root microbiota Viral crosslinking and solid-phase purification enables discovery of ribonucleoprotein complexes on incoming RNA virus genomes Mitochondrial single-cell ATAC-seq for high-throughput multi-omic detection of mitochondrial genotypes and chromatin accessibility Addendum: Micro-CT acquisition and image processing to track and characterize pulmonary nodules in mice Multiplexed single-cell proteomics using SCoPE2 查看全部 SARS-CoV-2 nsp3 macrodomain expression and purification protocol for crystallization expression purification ASAP CMD AViDD SARS-CoV-2 SARS-CoV-2 nsp3 SARS-CoV-2 nsp3 macrodomain SARS-CoV-2 macrodomain nsp3 macrodomain #nsp3 macrodomain mac1 his-tag Protocol for 3D bioprinting functional human brain tissues ASAPCRN A Supplemental Guide to Building the optoPlate-96 optoPlate-96 optoPlate optogenetics LEDs reflow soldering Optimized Protocol for RNA Extraction from Insect Samples Using TRIzol Reagent Smart-seq3xpress PCR and gel electropheresis Making Agarose for use in acute in vivo Electrophysiology Experiments Agarose Electrophysiology Neuropixels UPitt TriState SenNet TMC H&E staining histology staining Protocol for detection of Salmonella Typhi and Salmonella Paratyphi A in Surface water (and other types of water samples) Surface water water Salmonella Typhi Salmonella Paratyphi A enrichment detection environment Immunofluorescence Staining ASAPCRN 查看全部 STARR-seq and UMI-STARR-seq: Assessing Enhancer Activities for Genome-Wide-, High-, and Low-Complexity Candidate Libraries cis-regulatory element enhancer functional genomics gene expression gene regulation massively parallel reporter assay MPRA STARR-seq transcriptional regulation UMI-STARR-seq Measurement of Trogocytosis: Quantitative Analyses Validated with Rigorous Controls antibody-drug conjugates complement immune complex clearance immune adherence trogocytosis Production of Structured RNA Fragments by In Vitro Transcription and HPLC Purification biophysics HPLC RNA in vitro transcription sample preparation structural biology Optimized Workflow for Proteomics and Phosphoproteomics With Limited Tissue Samples limited protein phosphoprotein enrichment phosphoproteomics proteomics trigeminal ganglion Ploidy- and Purity-Adjusted Allele-Specific DNA Analysis Using CLONETv2 allele-specific analysis cancer genomics clonality ploidy purity Triplet-Primed PCR Assays for Accurate Screening of FMR1 CGG Repeat Expansion and Genotype Verification capillary electrophoresis FMR1 fragile X syndrome melting curve analysis screening triplet-primed PCR Preparation of Primary Rat Hepatocyte Spheroids Utilizing the Liquid-Overlay Technique bile canaliculi immunofluorescence liquid-overlay technique liver spheroids primary rat hepatocytes Generating Enteric Nervous System Progenitors from Human Pluripotent Stem Cells ENS progenitors human pluripotent stem cells hPSCs vagal neural crest Improving Species Level-taxonomic Assignment from 16S rRNA Sequencing Technologies 16S rRNA gene amplicons DADA2 microbiota composition metagenomics species-classification Investigating the Mitochondrial Permeability Transition Pore in Disease Phenotypes and Drug Screening calcium mitochondria permeability transition pore 查看全部
