Arabidopsis seeds priming
Tao-Ho Chang
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Abstract
The treated seeds can grow in medium or bulk soil to determine the impact of novel materials on plants.
Before start
Seed priming is an important method that increases the health of the plant.
Steps
Seeds sterilisation
The arabidopsis seeds are immersed with 50% bleach in 1.5 µL tubes.
100rpm
100rpm,28°C
Gently remove the supernatant and leave the seeds in the tube.
The sterile seeds are immersed in distilled water.
Repeat the process of step #3 to #5 for 10 times
Seeds treatment
The seeds were immersed in the specific concentration of treatment
-
Bacteria treatment: OD value to 0.1 and diluted 10 times.
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Fungus treatment: final concentration of spore suspension is 100 spores mL-1.
100rpm
150rpm
Wash seeds
The treated seeds are moved in a filter column with a collection tube.
100rpm,28°C
Remove the filter through in collection tube
Add 500 µL of distilled water to the filter column and resuspend the seeds.
Repeat the process of step #11 to #13 for 10 times
