Ancient DNA protocol collection – University of Tartu, Institute of Genomics

Ancient DNA protocols from sampling to library preparation for shotgun Illumina sequencing - aDNA lab UTIG. All protocols except the indexing PCR and purification need to be performed in a dedicated ancient DNA laboratory (see guidelines).

The individual steps are respective protocols are:

1. Sampling

1.a tooth roots

2. Decontamination of samples (optional)

3. Ancient DNA extraction

3.a high volume (chunk samples)

4. Purification of DNA extracts

5. Library preparation

6.a dsDNA, double indexing, non-UDG, 2x split

6.b dsDNA, single indexing, non-UDG

6.c dsDNA, double indexing, full-UDG, 2x split

6.d dsDNA, single indexing, full-UDG

7. Indexing PCR and purification