2022 featured protocols Admin User Published: 2022-12-15 DOI: 10.17504/protocols.io.q26g74nk3gwz/v1 AI 解读 浏览 1142 Abstract 2022 featured protocols Steps 推荐阅读 Nature Protocols Protocols IO Current Protocols Using high-resolution microscopy data to generate realistic structures for electromagnetic FDTD simulations from complex biological models Experimental modulation of physiological force application on leg joint neurons in intactDrosophila melanogaster Mitochondrial single-cell ATAC-seq for high-throughput multi-omic detection of mitochondrial genotypes and chromatin accessibility Identification, isolation and analysis of human gut-associated lymphoid tissues Development of RNA G-quadruplex (rG4)-targetingl-RNA aptamers by rG4-SELEX Self-assembled ultraflexible probes for long-term neural recordings and neuromodulation Hyperpolarized water as universal sensitivity booster in biomolecular NMR Improved sampling and DNA extraction procedures for microbiome analysis in food-processing environments An analytical workflow for dynamic characterization and quantification of metal-bearing nanomaterials in biological matrices A flow cytometry-based protocol for syngenic isolation of neurovascular unit cells from mouse and human tissues 查看全部 Human Neuronal Nucleus Isolation for Single-Nucleus Transcriptomic Profiling (10x Genomics) single-nucleus RNA-seq neuronal nucleus suspension human tissue spinal cord UAB-VU BIOMIC Preparation of Left Fresh Frozen Eye HuBMAP BIOMIC MSRC Vanderbilt UAB Eye Imaging eDNA extraction from buffer EtOH 100% using DNeasy Blood and Tissue Kit (QIAGEN) MERS-CoV Mpro fluorescence dose response The role of sphingolipids in the pathogenesis of psoriasis Psoriasis ceramide sphingolipid S1P-lyase Sphingosine Lipid signaling S1P-lyase Sphingosine 1-phosphate Sphingosine kinase Sphingomyelin Ceramide S1P receptor Detection of SARS CoV-2 in different samples with a singleplex and mutation resistant real time PCR assay SARS CoV-2 real time PCR Coronavirus pandemic outbreak Wuhan strain Omicron mutation TaME-seq2 integration library preparation illumina virus viral integrations snp snps variants whole genome sequencing genome genomics enrichment multiplex PCR Singleplex Assay for Fitness Measurements Deep mutational scanning protein sequence-function relationships fitness landscape laboratory automation SRA and Genbank BioSample-Linked Submission with Mercury_Prep and Mercury_Batch ACE-inhibitory activity assay: IC50 ACE angiotensin-converting enzyme antihypertensive IC50 bioactivity ngiotensin-I converting enzyme 查看全部 STARR-seq and UMI-STARR-seq: Assessing Enhancer Activities for Genome-Wide-, High-, and Low-Complexity Candidate Libraries cis-regulatory element enhancer functional genomics gene expression gene regulation massively parallel reporter assay MPRA STARR-seq transcriptional regulation UMI-STARR-seq Measurement of Trogocytosis: Quantitative Analyses Validated with Rigorous Controls antibody-drug conjugates complement immune complex clearance immune adherence trogocytosis Production of Structured RNA Fragments by In Vitro Transcription and HPLC Purification biophysics HPLC RNA in vitro transcription sample preparation structural biology Optimized Workflow for Proteomics and Phosphoproteomics With Limited Tissue Samples limited protein phosphoprotein enrichment phosphoproteomics proteomics trigeminal ganglion Ploidy- and Purity-Adjusted Allele-Specific DNA Analysis Using CLONETv2 allele-specific analysis cancer genomics clonality ploidy purity Triplet-Primed PCR Assays for Accurate Screening of FMR1 CGG Repeat Expansion and Genotype Verification capillary electrophoresis FMR1 fragile X syndrome melting curve analysis screening triplet-primed PCR Preparation of Primary Rat Hepatocyte Spheroids Utilizing the Liquid-Overlay Technique bile canaliculi immunofluorescence liquid-overlay technique liver spheroids primary rat hepatocytes Generating Enteric Nervous System Progenitors from Human Pluripotent Stem Cells ENS progenitors human pluripotent stem cells hPSCs vagal neural crest Improving Species Level-taxonomic Assignment from 16S rRNA Sequencing Technologies 16S rRNA gene amplicons DADA2 microbiota composition metagenomics species-classification Investigating the Mitochondrial Permeability Transition Pore in Disease Phenotypes and Drug Screening calcium mitochondria permeability transition pore 查看全部
