10x Protocols: Visium v2 CytAssist FFPE Deparaffinization, H&E staining, and Imaging-- University of Minnesota TMCs (CG000520 Rev B)
IOx Genomics, Laura J Niedernhofer, David A Bernlohr
Abstract
Protocols from 10x Genomics for Visium Spatial Gene Expression v2 chemistry on FPPE samples with the CytAssist component.
Protocol ID# (CG) and Revision used :
10x protocol CG000520, **Revision B** -- Deparaffinization, H&E staining, and Imaging prior to Library construction with CytAssist.
Note: These protocols may not be the current version offered by 10x Genomics but were used to produce the specific datasets connected to them. Please review the 10x Genomics support website for the most recent versions of the protocols prior to starting your experiment.
Steps
10x protocol CG000520, Revision B (Deparaffinization, H&E staining and Imaging prior to Library Construction and CytAssist)
10x CytAssist Visium Deparaffin HE, Rev B (Protocol CG000520).pdf
Additional Protocols/Resources
https://www.10xgenomics.com/support/spatial-gene-expression-ffpe
