10x Protocols: Chromium Single Cell/Nuclei Gene Expression Flex Multiplex-- University of Minnesota TMCs (CG000527 Rev C)
IOx Genomics, Laura Niedernhofer
Published: 2023-06-26 DOI: 10.17504/protocols.io.5jyl8jy4dg2w/v1
Abstract
10x Genomics Chromium Single Cell Expression flex protocol for library construction.
Protocol ID# (CG) and Revision letter provided here :
10x protocol CG000527, **Revision C** -- Library construction of 10x fixed single cell or nuclei
Note: These protocols may not be the current version offered by the company but were used to produce the specific datasets connected to them. Please review the company support websites for the most recent versions of the protocols prior to starting your experiment.
Steps
1.
2.
Complete single cell or nuclei isolation and 10x fixation prior to starting this protocol
3.
10x Protocol CG000527, Revision C (Library Construction):
10x Chromium Fixed RNA Profiling, Multiplexed Samples, Rev C (Protocol CG000527).pdf
