质粒库
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| 名称 | 描述 | 操作 |
|---|---|---|
| pET-Cas9-NLS-6xHis | Bacterial vector for regulated expression of S. pyogenes Cas9 with a C-terminal nuclear localization signal and 6xHis tag. | 查看 |
| pET-NLS-Cas9-6xHis | Bacterial vector for regulated expression of S. pyogenes Cas9 with an N-terminal nuclear localization signal and C-terminal 6xHis tag. | 查看 |
| pFGC-pcoCas9 | Binary plant vector for expressing plant codon-optimized Case9 from the 35SPPDK promoter. | 查看 |
| pGL3-U6-sgRNA-PGK-puromycin | - | 查看 |
| pgRNA-bacteria | Plasmid for expressing a single guide RNA (sgRNA) in bacteria. | 查看 |
| pgRNA-humanized | Lentiviral vector for expressing a guide RNA (gRNA) together with a puromycin resistance marker and mCherry. | 查看 |
| pGuide | - | 查看 |
| pGuide-it-sgRNA1 Vector (Linear) | Linearized CRISPR/Cas9 vector with an improved scaffold, for cloning and expression of a single guide RNA (sgRNA). | 查看 |
| pGuide-it-tdTomato Vector (Linear) | Linearized CRISPR/Cas9 vector with a tdTomato marker, for cloning and expression of a single guide RNA (sgRNA). | 查看 |
| pGuide-it-ZsGreen1 Vector (Linear) | Linearized CRISPR/Cas9 vector with a ZsGreen1 marker, for cloning and expression of a single guide RNA (sgRNA). | 查看 |
| pHAGE EF1-alpha dCas9-KRAB | Lentiviral CRISPR interference vector for constitutive expression of catalytically inactive dCas9 fused to a KRAB transcriptional repressor domain. | 查看 |
| pHAGE EF1-alpha dCas9-VP64 | Lentiviral CRISPR activation vector for constitutive expression of catalytically inactive dCas9 fused to the VP64 transcriptional activation domain. | 查看 |
| pHAGE-TO-dCas9-3XGFP | Lentiviral vector for labeling chromosomal loci by tetracycline-regulatable expression of catalytically inactive dCas9 fused to three copies of GFP. | 查看 |
| pHAGE-TO-dCas9-3XmCherry | Lentiviral vector for labeling chromosomal loci by tetracycline-regulatable expression of catalytically inactive dCas9 fused to three copies of mCherry. | 查看 |
| pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2 | Lentiviral vector for labeling chromosomal loci by tetracycline-regulatable expression of catalytically dead Streptococcus thermophilus dCas9 fused to three copies of mTagBFP2. | 查看 |
| pHAGE-TO-nmdCas9-3XGFP | Lentiviral vector for labeling chromosomal loci by tetracycline-regulatable expression of catalytically dead Neisseria meningitidis dCas9 fused to three copies of GFP. | 查看 |
| pHAGE-TO-nmdCas9-3XmCherry | Lentiviral vector for labeling chromosomal loci by tetracycline-regulatable expression of catalytically dead Neisseria meningitidis dCas9 fused to three copies of mCherry. | 查看 |
| pHAGE TRE dCas9 | Lentiviral CRISPR activation vector for doxycycline-inducible expression of catalytically inactive dCas9. | 查看 |
| pHAGE TRE dCas9-KRAB | Lentiviral CRISPR interference vector for doxycycline-inducible expression of catalytically inactive dCas9 fused to a KRAB transcriptional repressor domain. | 查看 |
| pHAGE TRE dCas9-VP64 | Lentiviral CRISPR activation vector for doxycycline-inducible expression of catalytically inactive dCas9 fused to the VP64 transcriptional activation domain. | 查看 |
