质粒库
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筛选
| 名称 | 描述 | 操作 |
|---|---|---|
| pLenti-Cas9-P2A-tGFP | 3rd-generation lentiviral vector for expressing Cas9 and TurboGFP under control of the CMV promoter. | 查看 |
| pLenti-Cas-Guide | - | 查看 |
| pLenti-EF1a-Cas9-IRES-Puro | 3rd-generation lentiviral vector for expressing Cas9 and a puromycin resistance marker under control of the EF-1α promoter. | 查看 |
| pLenti-Guide-Puro | - | 查看 |
| pLKO.1-puro U6 sgRNA BfuAI stuffer | - | 查看 |
| pLSC-5 | - | 查看 |
| pLV hUbC-Cas9-T2A-GFP | Lentiviral vector for co-expression of human codon-optimized Cas9 and enhanced GFP (EGFP). | 查看 |
| pLV hUbC-dCas9-T2A-GFP | Lentiviral vector for expressing enhanced GFP (EGFP) together with catalytically inactive dCas9. | 查看 |
| pLV hUbC-dCas9 VP64-T2A-GFP | Lentiviral vector for expressing enhanced GFP (EGFP) together with catalytically inactive dCas9 fused to the VP64 transcriptional activation domain. | 查看 |
| pLVX-hyg-sgRNA1 Vector (Linear) | Linearized lentiviral vector with a hygromycin resistance marker, for cloning and expression of a single guide RNA (sgRNA). | 查看 |
| pLVX-puro-Cas9 | Lentiviral Cas9 expression vector with the constitutive CMV promoter and a puromycin resistance marker. | 查看 |
| pLX-sgRNA | - | 查看 |
| pMB66 | C. elegans plasmid for expression of nematode codon-optimized Cas9 fused to monomeric EGFP and nuclear localization signals. | 查看 |
| pMB70 | C. elegans plasmid for expressing a single guide RNA (sgRNA). | 查看 |
| pME-Cas9 | Middle entry Gateway® vector encoding zebrafish codon-optimized S. pyogenes Cas9 with nuclear localization signals. | 查看 |
| pME-Cas9-T2A-GFP | Middle entry Gateway® vector encoding zebrafish codon-optimized S. pyogenes Cas9 with nuclear localization signals and with a C-terminal GFP. | 查看 |
| pMJ806 | pET-derived bacterial vector for expressing 6xHis- and MBP-tagged S. pyogenes Cas9. | 查看 |
| pMJ841 | pET-derived bacterial vector for expressing 6xHis- and MBP-tagged catalytically inactive S. pyogenes Cas9. | 查看 |
| pMJ920 | Doudna lab plasmid for mammalian cell expression of human codon-optimized Cas9 together with GFP. | 查看 |
| pMLM3705 | CRISPR activation plasmid for mammalian cell expression of catalytically inactive dCas9 fused to the VP64 transcriptional activation domain. | 查看 |
