质粒库
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| 名称 | 描述 | 操作 |
|---|---|---|
| 3xFLAG-dCas9 pCMV-7.1 | Plasmid for expression in mammalian cells of FLAG®-tagged catalytically inactive dCas9, for engineered ChIP (enChIP) purification of specific genomic regions. | 查看 |
| 3xFLAG-dCas9 pMXs-neo | Retroviral vector with a G418 resistance marker, encoding FLAG®-tagged dCas9 for engineered ChIP (enChIP) purification of specific genomic regions. | 查看 |
| 3xFLAG-dCas9 pMXs-puro | Retroviral vector with a puromycin resistance marker, encoding FLAG®-tagged dCas9 for engineered ChIP (enChIP) purification of specific genomic regions. | 查看 |
| Cas9m4 | Plasmid for expression in mammalian cells of catalytically dead Cas9m4. | 查看 |
| Cas9m4-VP64 | Plasmid for expression in mammalian cells of catalytically dead Cas9m4 fused to the VP64 transcriptional activation domain. | 查看 |
| CRISPRi library backbone | Backbone vector for generating a CRISPR-mediated transcriptional repression (CRISPRi) library. | 查看 |
| dCAS9-VP64_GFP | Zhang lab lentiviral vector encoding enhanced GFP (EGFP) plus catalytically inactive dCas9 fused to the VP64 transcriptional activation domain. | 查看 |
| eSpCas9(1.1) | - | 查看 |
| GeneArt CRISPR Nuclease Vector - CD4 (linearized) | All-in-one CRISPR/Cas9 vector for genome editing, with a CD4 reporter for bead-based cell isolation. | 查看 |
| GeneArt CRISPR Nuclease Vector - OFP (linearized) | All-in-one CRISPR/Cas9 vector for genome editing, with an orange fluorescent protein reporter. | 查看 |
| gRNA_AAVS1-T1 | Plasmid for guide RNA (gRNA) targeting of an AAVS1 fragment interrupting an integrated GFP gene in human cells, using the AAVS1 T1 target sequence. | 查看 |
| gRNA_AAVS1-T2 | Plasmid for guide RNA (gRNA) targeting of an AAVS1 fragment interrupting an integrated GFP gene in human cells, using the AAVS1 T2 target sequence. | 查看 |
| gRNA_Cloning Vector | Mammalian cell plasmid for generating a guide RNA (gRNA) expression cassette by oligo synthesis and Gibson assembly. | 查看 |
| gRNA_GFP-T1 | Plasmid for guide RNA (gRNA) targeting of an integrated GFP gene in human cells using the GFP T1 target sequence. | 查看 |
| gRNA_GFP-T2 | Plasmid for guide RNA (gRNA) targeting of an integrated GFP gene in human cells using the GFP T2 target sequence. | 查看 |
| HBT-pcoCas9 | Plasmid for transient expression of plant codon-optimized Cas9 in plant cells. Use together with pUC119-gRNA. | 查看 |
| hCas9 | Mammalian vector with a G418 resistance marker for expressing human codon-optimized Cas9. | 查看 |
| hCas9_D10A | Mammalian vector with a G418 resistance marker for expressing human codon-optimized Cas9 with the D10A nickase mutation. | 查看 |
| JDS246 | Plasmid for expressing FLAG®-tagged human codon-optimized Cas9 in mammalian cells. | 查看 |
| lentiCas9-Blast | Zhang lab lentiviral vector with a blasticidin resistance marker, also known as pLentiCas9-Blast, for expressing Cas9. Use together with lentiGuide-Puro. | 查看 |