质粒库
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| 名称 | 描述 | 操作 |
|---|---|---|
| pSQT834 | Plasmid for mammalian cell expression of the Csy4 CRISPR-associated endoribonuclease and wild-type S. pyogenes Cas9. | 查看 |
| pST1374-NLS-flag-linker-Cas9 | Plasmid for mammalian cell expression of Cas9 with an N-terminal nuclear localization signal followed by a FLAG® tag. | 查看 |
| pST1374-N-NLS-flag-linker-Cas9-D10A | Plasmid for mammalian cell expression of Cas9 with the D10A nickase mutation and with an N-terminal nuclear localization signal followed by a FLAG® tag. | 查看 |
| pST1374-N-NLS-flag-linker-Cas9-H840A | Plasmid for mammalian cell expression of Cas9 with the H840A nickase mutation and with an N-terminal nuclear localization signal followed by a FLAG® tag. | 查看 |
| pT3TS-nCas9n | Plasmid for T3 promoter-driven expression of zebrafish codon-optimized S. pyogenes Cas9. | 查看 |
| pT7-Cas9 | In vitro transcription vector with the T7 promoter, for producing myc- and FLAG®-tagged S. pyogenes Cas9. | 查看 |
| pT7-Cas9-Nickase | In vitro transcription vector with the T7 promoter, for producing myc- and FLAG®-tagged S. pyogenes Cas9 with the D10A nickase mutation. | 查看 |
| pT7-gRNA | Plasmid for in vitro expression of a single guide RNA (sgRNA) using the T7 promoter. | 查看 |
| pT7-Guide-IVT | In vitro transcription vector with the T7 promoter, for producing a guide RNA (gRNA). | 查看 |
| pTDH3-dCas9 | Yeast centromeric vector for expressing catalytically inactive dCas9 with the TDH3 (glyceraldehyde 3-phosphate dehydrogenase) promoter. | 查看 |
| pTDH3-dCas9-Mxi1 | Yeast centromeric vector for expressing catalytically inactive dCas9 fused to the Mxi1 transcriptional repressor domain. | 查看 |
| pTOL-hCMV-TET3G-Hygro Transactivator | Lentiviral vector with a hygromycin resistance marker for expression of the Tet-On® 3G doxycycline-dependent transactivator. | 查看 |
| pTPGI_dCas9_VP64 | Yeast plasmid for inducible expression of catalytically inactive S. pyogenes dCas9 fused to the VP64 transcriptional activation domain. | 查看 |
| pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B | - | 查看 |
| pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E4orf6 | - | 查看 |
| pU6-BbsI-chiRNA | Plasmid for expressing a chimeric RNA in Drosophila cells from the U6 promoter. Use together with pHsp70-Cas9. | 查看 |
| PU6_klp-12_sgRNA | C. elegans plasmid for expressing a single guide RNA (sgRNA). Use together with Peft-3::cas9-SV40_NLS::tbb-2 3''UTR. | 查看 |
| pUC119-gRNA | CRISPR plasmid for expressing a single guide RNA (sgRNA) in plant cells. Use together with HBT-pcoCas9. | 查看 |
| pUC57-sgRNA expression vector | Plasmid for in vitro transcription of a single guide RNA (sgRNA) using the T7 promoter. | 查看 |
| Puro-Cas9 donor | Vector for targeting a tetracycline-inducible Cas9 cassette to the adeno-associated virus integration site (AAVS1) in the human PPP1R12C gene. | 查看 |
