质粒库
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| 名称 | 描述 | 操作 |
|---|---|---|
| pCRCT | High-copy yeast plasmid for disrupting genes by expressing S. pyogenes Cas9 together with a tracrRNA and one or more CRISPR RNAs (crRNAs). | 查看 |
| pCRISPomyces-1 | Streptomyces plasmid for expression of codon-optimized Cas9, tracrRNA, and custom crRNA. | 查看 |
| pCRISPomyces-2 | Streptomyces plasmid for expression of codon-optimized Cas9 and a single guide RNA (sgRNA). | 查看 |
| pCRISPR | Plasmid for reconstituting the S. pyogenes CRISPR/Cas system in E. coli. Use together with pCas9. | 查看 |
| pCRISPRa-Enhancer | Mammalian gene activation vector for expressing an MS2-p65-HSF1 fusion. Use together with pCas-Guide-CRISPRa. | 查看 |
| pCS2-Cas9 | S. pyogenes Cas9 plasmid for in vivo expression or in vitro mRNA production. | 查看 |
| pCS2+hSpCas9 | Plasmid for in vitro transcription of human codon-optimized S. pyogenes Cas9 using the SP6 promoter. | 查看 |
| pCS2-nCas9n | Plasmid for expressing zebrafish codon-optimized S. pyogenes Cas9 with N- and C-terminal nuclear localization signals. | 查看 |
| pCW-Cas9 | Lentiviral vector for doxycycline-inducible expression of FLAG®-tagged Cas9. | 查看 |
| pdCas9 | Bacterial plasmid for expressing catalytically inactive dCas9 together with a crRNA precursor, for transcriptional repression. | 查看 |
| pdCas9-bacteria | Bacterial plasmid for tetracycline-inducible expression of catalytically dead S. pyogenes Cas9. | 查看 |
| pdCas9 BFP-humanized | Retroviral vector encoding human codon-optimized catalytically dead dCas9 fused to TagBFP, for visualizing genomic loci using a small guide RNA (sgRNA). | 查看 |
| pdCas9-humanized | Retroviral vector encoding human codon-optimized catalytically dead dCas9 for CRISPR interference. | 查看 |
| pDCC6 | Drosophila CRISPR/Cas9 genome editing vector for expressing a single guide RNA (sgRNA) together with Cas9. | 查看 |
| pDD162 (Peft-3_Cas9 + Empty sgRNA) | C. elegans plasmid for expressing nematode codon-optimized Cas9 together with a single guide RNA (sgRNA). | 查看 |
| pDR274 | Plasmid for in vitro transcription of a single guide RNA (sgRNA) using the T7 promoter. Archived by Addgene as DR274. | 查看 |
| Peft-3_cas9-SV40_NLS_tbb-2 3'UTR | C. elegans plasmid for expressing nematode codon-optimized Cas9 with a synthetic intron. Use together with PU6::klp-12_sgRNA. | 查看 |
| pET28a Cas9-Cys | Bacterial plasmid for regulated expression of S. pyogenes Cas9 with a C-terminal cysteine. | 查看 |
| pET-28b-Cas9-His | Bacterial plasmid for expressing a 6xHis-tagged Cas9 protein, which can be purified and microinjected for gene editing. | 查看 |
| pET-Cas9-6xHis | Bacterial vector for regulated expression of S. pyogenes Cas9 with a 6xHis tag. | 查看 |
