immunehistochemistry and transcriptomic analysis of iodine and breast cancer
Carmen Aceves
Abstract
The present work analyzes the antineoplastic effects of molecular iodine alone and in combination with the neoadjuvant therapy FEC/TE (5-fluorouracil/epirubicin/cyclophosphamide or taxotere/epirubicin) in women with breast cancer. In this protocol the immunohistochemistry methodology for the quantification of the estrogen receptor and the proteins associated with the installation of the mesenchymal epithelium transition (e-cadherin and vimentin), immune response (CD8) and cell death (TUNEL) is described, and the methodology for transcriptomic analyses includes: RNA-Seq and transcriptomic analysis; pathway enrichment analysis; validation of the immune response and epithelium/stroma rate and, prediction of the transcription factor regulatory network and peroxisome proliferator-activated receptors type gamma (PPARg) interaction
