WATER PRODUCTION FOR AWARE (Metals)

The water production for AWARE main activities includes three stages – disinfection by ultraviolet C radiation (UVC), storage for12h 0m 0s-24h 0m 0s(according to water load and season) and ozonation. The water quality is monitored at these three stages, for the parameters indicated in Figure 1 below.

Sampling, Processing, and Analyses

Water samples are collected (see Figure 2) and processed within a6h 0m 0sinterval, before being shipped for the partner responsible for the analyses (Table 1). In case no processing is needed, samples are frozen and stored at-80°Cwithin3h 0m 0s.

For each sampling event, the date, day of the week and hour; the temperature and rain. Sampling points, indicated in Figure 2 were designated from A to I:

• Influent of primary treatment (A)

• Influent of biological treatment (activated sludge) (B)

• Treated secondary effluent (C)

• Sand filter effluent (D)

• UVC effluent (E)

• Storage for reuse tank effluent (F)

• Ozonation effluent (1 dose, e.g.,5mgO3) - MITO3X technology - (G)

• Effluent of the vacuum UV oxidation (VUV) (H)

• Effluent of reactive storage / Influent of the recirculation aquaculture system (RAS) (I)

Metals: Metals:

Analysis: Detection and quantification of metals

Method: Acidified water samples were analyzed for several metals (e.g. Al, Cu, Ni, Co, Pb, Zn, Cd, Pt, Sb, Sn, U, As, Ag, Hg, REEs) by means of ICP-MS (7900 Agilent) using matrix-matched external calibration.

Observations: Samples were filtered/centrifuged within12h 0m 0safter collection, and then acidified to 0.15M HNO3.

Using the EU Drinking Water Directive:

Metals - DIRECTIVE 2008/105/EC OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL of 16 December 2008 on environmental quality standards in the field of water policy