Tutorial: design, production and testing of oncolytic viruses for cancer immunotherapy

Shashi Gujar, Jonathan G. Pol, Vishnupriyan Kumar, Manuela Lizarralde-Guerrero, Prathyusha Konda, Guido Kroemer, John C. Bell

Published: 2024-05-19 DOI: 10.1038/s41596-024-00985-1

Oncolytic viruses

Cancer immunotherapy

Preclinical testing

Viral design

Anticancer potential

AI 解读

Genetic manipulation and immortalized culture of ex vivo primary human germinal center B cells

The cell-line-derived subcutaneous tumor model in preclinical cancer research

Subcellular patch-clamp techniques for single-bouton stimulation and simultaneous pre- and postsynaptic recording at cortical synapses

NovoSpaRc: flexible spatial reconstruction of single-cell gene expression with optimal transport

Genome-wide pooled CRISPR screening in neurospheres

High-throughput cultivation and identification of bacteria from the plant root microbiota

Viral crosslinking and solid-phase purification enables discovery of ribonucleoprotein complexes on incoming RNA virus genomes

Mitochondrial single-cell ATAC-seq for high-throughput multi-omic detection of mitochondrial genotypes and chromatin accessibility

Addendum: Micro-CT acquisition and image processing to track and characterize pulmonary nodules in mice

Multiplexed single-cell proteomics using SCoPE2

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Sections

Figures

References

Overview of the tutorial
Design, selection and engineering of OVs
- Selection of OV platform
Propagation, purification and titration of OVs
In vivo preclinical testing of OVs
Concluding remarks
- Challenges
- Future directions
Acknowledgements
Table 2 Replication-competent RNA viruses with oncolytic activity.

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Tutorial: design, production and testing of oncolytic viruses for cancer immunotherapy

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