Tissue Sectioning Guidelines (FFPE) - CODEX/PhenoCycler

Prepare the microtome for sectioning. Section the tissue at a thickness of 5 μm .

For best results, the tissue should be completely adhered to the slide with minimal tears or folds.

Gently place the tissue section in the center of the slide within the imageable area as shown in

grey rectangle below.

If tissue overlaps the boundary, proper coverslip seal will not be formed. Multiple tissues can be placed within the boundary, as long as there is no overlap of paraffin over adjacent tissues

Example of good tissue placement:

Dry tissue sections overnight at RT

After drying, store cut slides at 4°C (for up to six months)

NOTE: Do not place any stickers or labels on the frosted end of the slide as it may interfere with coverslip adhesion. Use markers or directly print labels on the slide.