Tissue H&E Staining | HuBMAP | JHU-TMC

The following are guidelines that will have an effective staining window of 2 to 5 minutes. Developed as progressive stains, the intensity of nuclear staining will increase as the time increases. The hematoxylin and eosin stains will have an expected throughput of 2,000-2,500 slides per 500mL bottle. Actual results may vary from lab to lab depending on the staining equipment used, control of carry over into each solution, and length of time stains are left exposed. As a general rule, we recommend changing the hematoxylin and eosin stains once per week if throughput has not been reached. Clarifying and Bluing solutions should be changed more often.

The Leica autostainer is a single fully automated integrated stainer used for standard

H&E staining and operates by progressing each slide through a series of chemical

changes that first deparaffinize and then stains the tissue slides for histologic review. To

use, the machine is powered on and the covers are removed from the staining vessels

every morning and the hematoxylin is filtered daily. The daily number of slides stained is

added to the log sheet beside the stainer, and all the solutions are changed when the

slide tally reaches 250. At the end of the day, the staining vessels are then covered and

the machine is powered off