SynGO analysis
Chuyu Chen
Published: 2024-06-17 DOI: 10.17504/protocols.io.x54v92r94l3e/v1
Abstract
synapse function and gene enrichment analysis
Steps
SynGO analysis
1.
Convert mouse Gene ID to human genes symbols with ID conversion tool
2.
Copy converted genes symbols to the SynGO input section, select background as brain expressed, and name the experiment in the description
3.
Start analysis and export data to Excel files
4.
Open custom color-coding of SynGO ontologies
5.
Provide GO term ID and -log10(GSEA 'gene cluster' FDR corrected p-value) in the input section, label for figure legend, and three values to create a color gradient suitable for the data.
6.
Open a color-picker to change the color-coding of the data
7.
Download figures
