Stress-free single-cell transcriptomic profiling and functional genomics of murine eosinophils

Costanza Borrelli, Alessandra Gurtner, Isabelle C. Arnold, Andreas E. Moor

Published: 2024-03-19 DOI: 10.1038/s41596-024-00967-3

CRISPR screening

single-cell RNA sequencing

eosinophils

granulocytes

intestinal subsets

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Supplementary information

Supplementary Information

Supplementary Figs. 1–3.

Reporting Summary

Supplementary Table 1

Sequences for the primers used in the protocol.

Genetic manipulation and immortalized culture of ex vivo primary human germinal center B cells

The cell-line-derived subcutaneous tumor model in preclinical cancer research

Subcellular patch-clamp techniques for single-bouton stimulation and simultaneous pre- and postsynaptic recording at cortical synapses

NovoSpaRc: flexible spatial reconstruction of single-cell gene expression with optimal transport

Genome-wide pooled CRISPR screening in neurospheres

High-throughput cultivation and identification of bacteria from the plant root microbiota

Viral crosslinking and solid-phase purification enables discovery of ribonucleoprotein complexes on incoming RNA virus genomes

Mitochondrial single-cell ATAC-seq for high-throughput multi-omic detection of mitochondrial genotypes and chromatin accessibility

Addendum: Micro-CT acquisition and image processing to track and characterize pulmonary nodules in mice

Multiplexed single-cell proteomics using SCoPE2

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Stress-free single-cell transcriptomic profiling and functional genomics of murine eosinophils

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