Single-molecule magnetic tweezers to probe the equilibrium dynamics of individual proteins at physiologically relevant forces and timescales

Rafael Tapia-Rojo, Marc Mora, Sergi Garcia-Manyes

Published: 2024-03-11 DOI: 10.1038/s41596-024-00965-5

Extended

Extended Data Fig. 1 Calculating the stiffness of the magnetic trap.

Stiffness of the magnetic trap created by the N52 magnets (voice-coil configuration) ( a ) and magnetic tape head ( b ). The magnetic trap stiffnesses can be simply calculated as dF/dz , where z is the distance between the gap (magnets or tape head) and the magnetic bead. Because of the nonlinearity of F ( z ), the stiffness changes over the control parameter (magnet position or electric current), but in the operating regime of the trap this results in a very soft trap (~10 −4 pN/nm), resulting in effective force clamp conditions (no appreciable change in force over the range in which the bead moves).

Extended Data Fig. 2 Calibration of the tweezers.

Calibration of the voice coil-based ( a ) or tape head–based ( b ) magnetic tweezers using the worm-like chain model for polymer elasticity (left) and comparison of the calibration using the worm-like chain (WLC) and freely jointed chain (FJC) (right). The FJC gives a lower contour length ( ΔL c = 16.3 nm) compared to the WLC ( ΔL c = 18.6 nm). All error bars are s.d.

Extended Data Fig. 3 Tape head and magnets.

The magnetic tape head and voice-coil-mounted permanent magnets with a magnification of the gap region.

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Single-molecule magnetic tweezers to probe the equilibrium dynamics of individual proteins at physiologically relevant forces and timescales

Extended

Supplementary information

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