Recordings of cervical vagus nerve activity
Vitaly Napadow, Ilknur Ay, Catherine Morello
Abstract
The goal of this work is to evaluate laterality of vagus nerve efference evoked by unilateral auricular branch of the vagus nerve (ABVN) stimulation by performing bilateral recordings of cervical vagus nerve activity (CVNA).
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Ten-to-12 week old male and female Wistar rats ranging in weight from 232-400g were anesthesized.
Stimulation was delivered to cymba and cavum conchae using a Grass S48 stimulator in conjunction with a Grass stimulus isolation unit and constant current unit stimulator.
CVNA was recorded using hook electrodes from the right and/or left cervical vagus nerve in response to left auricular vagus nerve stimulation.
ABVN stimulation protocol was as follows: three 0.5mA trains of stimulation (1Hz, 0.5ms) repeated at every 5min followed by three 1mA trains of stimulation (1Hz, 0.5ms) repeated at every 5 min. Parameters for ABVN stimulation included a series of 5 stimulus pulse trains, with each pulse consisting of a 0.5 mA current intensity, 0.5 ms pulse width, and delivered at a frequency of 20Hz.
