Quantitative determination of phosphatidylethanol in dried blood spots for monitoring alcohol abstinence Marc Luginbühl, Frederike Stöth, Alexandra Schröck, Stefan Gaugler, Wolfgang Weinmann Published: 2020-12-06 DOI: 10.1038/s41596-020-00416-x AI 解读 浏览 1140 PDFMetadataSupplementary information Reporting Summary 推荐阅读 Nature Protocols Protocols IO Current Protocols Genetic manipulation and immortalized culture of ex vivo primary human germinal center B cells The cell-line-derived subcutaneous tumor model in preclinical cancer research Subcellular patch-clamp techniques for single-bouton stimulation and simultaneous pre- and postsynaptic recording at cortical synapses NovoSpaRc: flexible spatial reconstruction of single-cell gene expression with optimal transport Genome-wide pooled CRISPR screening in neurospheres High-throughput cultivation and identification of bacteria from the plant root microbiota Viral crosslinking and solid-phase purification enables discovery of ribonucleoprotein complexes on incoming RNA virus genomes Mitochondrial single-cell ATAC-seq for high-throughput multi-omic detection of mitochondrial genotypes and chromatin accessibility Addendum: Micro-CT acquisition and image processing to track and characterize pulmonary nodules in mice Multiplexed single-cell proteomics using SCoPE2 查看全部 SARS-CoV-2 nsp3 macrodomain expression and purification protocol for crystallization expression purification ASAP CMD AViDD SARS-CoV-2 SARS-CoV-2 nsp3 SARS-CoV-2 nsp3 macrodomain SARS-CoV-2 macrodomain nsp3 macrodomain #nsp3 macrodomain mac1 his-tag Protocol for 3D bioprinting functional human brain tissues ASAPCRN A Supplemental Guide to Building the optoPlate-96 optoPlate-96 optoPlate optogenetics LEDs reflow soldering Optimized Protocol for RNA Extraction from Insect Samples Using TRIzol Reagent Smart-seq3xpress PCR and gel electropheresis Making Agarose for use in acute in vivo Electrophysiology Experiments Agarose Electrophysiology Neuropixels UPitt TriState SenNet TMC H&E staining histology staining Protocol for detection of Salmonella Typhi and Salmonella Paratyphi A in Surface water (and other types of water samples) Surface water water Salmonella Typhi Salmonella Paratyphi A enrichment detection environment Immunofluorescence Staining ASAPCRN 查看全部 RNA Extraction from Plant Tissue with Homemade Acid Guanidinium Thiocyanate Phenol Chloroform (AGPC) AGPC home-made buffers molecular biology protocol multiple plant species and tissues nucleic acids RNA isolation Genotyping Microbial Communities with MIDAS2: From Metagenomic Reads to Allele Tables copy number variant genotyping microbiome single nucleotide variant strain MIDAS2 Using ExpressAnalyst for Comprehensive Gene Expression Analysis in Model and Non-Model Organisms differential expression analysis meta-analysis proteomics transcriptomics visual analytics Supervised Learning of Gene Regulatory Networks gene expression profiles gene regulatory networks supervised learning support vector machine One-Flask Synthesis of Cyclic Diguanosine Monophosphate (c-di-GMP) bacterial signaling c-di-GMP chemical synthesis Antibody-Based CAR T Cells Produced by Lentiviral Transduction CAR T cells chimeric antigen receptor lentiviral transduction Two-Tailed RT-qPCR for the Quantification of A-to-I-Edited microRNA Isoforms A-to-I editing biomarker discovery microRNA non-coding RNAs RNA editing two-tailed RT-qPCR Specific Localization and Quantification of the Oligo-Mouse-Microbiota (OMM12) by Fluorescence In Situ Hybridization (FISH) altered Schaedler flora intestine microbial ecology oligo-MM sDMDMm2 single-cell analysis spatial structure Optical Genome Mapping for Applications in Repeat Expansion Disorders optical genome mapping repeat expansion disorders somatic instability structural variant analysis whole genome analysis Single-Molecule Localization Microscopy Using Time-Lapse Imaging of Single-Antibody Labeling antibodies multiplexed imaging single-antibody labeling single-molecule localization microscopy superresolution imaging time-lapse imaging 查看全部