Protocol for Exo-CIP™ Rapid PCR Cleanup (#E1050)
New England Biolabs
Published: 2022-02-11 DOI: 10.17504/protocols.io.bg9xjz7n
Abstract
Exo-CIP™ Rapid PCR Cleanup Kit
- Rapidly degrade residual PCR primers and dephosphorylate excess dNTPs after amplification
- Reaction complete in 4 minutes
- Thermolabile formulation can be heat inactivated in 1 minute at 80°C
- PCR product can be used directly in downstream applications
- Compatible with commonly-used reaction buffers
Steps
1.
Transfer 5µL to a new PCR tube and add 1µL and 1µL. The final volume is 7µL.
2.
Mix thoroughly and briefly centrifuge at 1000x g.
3.
Incubate the reaction tube for 0h 4m 0s at 37°C followed by 0h 1m 0s at 80°C.
4. * A simple way to determine the amount of your amplicon is to load 3µL on an agarose gel along with a known amount of a control DNA for comparison. Alternatively, direct measurement using fluorescent dye based kit (e.g., Qubit™) will ensure the proper amount of DNA is submitted.
Submit 3µL or less* for sequencing using BigDye™ Terminator v3.1 Cycle Sequencing Kit or store the treated samples at -20°C for longer term storage.
Note
