ProBac-seq, a bacterial single-cell RNA sequencing methodology using droplet microfluidics and large oligonucleotide probe sets
Prosenjit Samanta, Samuel F. Cooke, Ryan McNulty, Sahand Hormoz, Adam Rosenthal
Published: 2024-05-19 DOI: 10.1038/s41596-024-01002-1
cellular heterogeneity
bacterial single-cell RNA sequencing
ProBac-seq
oligonucleotide probe sets
droplet microfluidics




























Supplementary information
Supplementary Information
Supplementary Figs. 1–6.
Supplementary Table 1
B. subtilis probes ordered from Twist Bioscience and shown here as a representation of an initial probe sequence
Supplementary Table 2
Primers for probe amplification and library generation. RCA primers shown here are specific for B. subtilis