ProBac-seq, a bacterial single-cell RNA sequencing methodology using droplet microfluidics and large oligonucleotide probe sets

Prosenjit Samanta, Samuel F. Cooke, Ryan McNulty, Sahand Hormoz, Adam Rosenthal

Published: 2024-05-19 DOI: 10.1038/s41596-024-01002-1

cellular heterogeneity

bacterial single-cell RNA sequencing

ProBac-seq

oligonucleotide probe sets

droplet microfluidics

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Supplementary information

Supplementary Information

Supplementary Figs. 1–6.

Supplementary Table 1

B. subtilis probes ordered from Twist Bioscience and shown here as a representation of an initial probe sequence

Supplementary Table 2

Primers for probe amplification and library generation. RCA primers shown here are specific for B. subtilis

Genetic manipulation and immortalized culture of ex vivo primary human germinal center B cells

The cell-line-derived subcutaneous tumor model in preclinical cancer research

Subcellular patch-clamp techniques for single-bouton stimulation and simultaneous pre- and postsynaptic recording at cortical synapses

NovoSpaRc: flexible spatial reconstruction of single-cell gene expression with optimal transport

Genome-wide pooled CRISPR screening in neurospheres

High-throughput cultivation and identification of bacteria from the plant root microbiota

Viral crosslinking and solid-phase purification enables discovery of ribonucleoprotein complexes on incoming RNA virus genomes

Mitochondrial single-cell ATAC-seq for high-throughput multi-omic detection of mitochondrial genotypes and chromatin accessibility

Addendum: Micro-CT acquisition and image processing to track and characterize pulmonary nodules in mice

Multiplexed single-cell proteomics using SCoPE2

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ProBac-seq, a bacterial single-cell RNA sequencing methodology using droplet microfluidics and large oligonucleotide probe sets

Supplementary information

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