Preparation of Agarose Gel
NUS iGEM
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Abstract
2023 NUS-Singapore iGEM team followed this protocol to prepare a 1% agarose gel. The team used this 1% agarose gel to isolate the DNA fragments from the common PCR and the colony PCR products.
Steps
For a 1% agarose gel, mix the following in a conical flask:
5gof Agarose powder.50mLof 1x TAE buffer (Tris-Acetate-EDTA Buffer).
Swirl the agarose solution to mix it well.
Heat the agarose solution in a microwave until it boils.
Take out the conical flask from the microwave and swirl the conical flask until the agarose solution is clear and without undissolved agarose powder or lumps.
Add 5µL of Ultra GelRed gel stain into the agarose solution and swirl the conical flask until the colour becomes uniform.
Secure a gel tray tightly onto a gel caster and place an 8-well comb or 15-well comb into the tray.
Pour the agarose solution into the tray, ensuring that there are no bubbles.
Cool down the agarose solution for at least 0h 30m 0s to get a solidified agarose gel.
