Nasal Lavage Sample collection and processing
Mary Xylaki
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Abstract
This protocol describes the nasal lavage fluid sampling that can be used for Omics (for Proteome analysis, see Schoenebeck et al, 2015; for Microbiome analysis see Heintz-Buschart et al, 2017) or RT-QuIC. Collection proceedure was adapted from B. Schoenebeck et al. 2015 (doi:10.1016/j.bbapap.2015.01.015).
Steps
Nasal Lavage Sample collection
Two nasal lavage samples (NLS) are collected per person in each step, one from each nostril.
Both nasal cavities are pre-washed with 2 mL of physiological NaCl solution with the use of a plastic disposable pipette to remove bacterial impurities.
After 5 mins of incubation, each nasal cavity is rinsed again with 2 mL physiological NaCl solution with subsequent collection of the NLS into a tube.
Following this step, an additional rinse is performed with 2 mL NaCl 10% (w/v) aqeous solution and collection of the NLS into a tube.
A final rinse is performed with 2 mL NaCl 20% (w/v) aqeous solution and collection of the NLS into a tube.
Depending on downstream applications, NLS samples can be immediatelly frozen at -80 °C until analysis or centrifuged at 10.000 x g for 5 mins at 4 °C in order to remove cellullar debri and then stored.
