LAB Agarose Gel Electrophoresis Buffer Recipe
Brian Teague
Published: 2022-07-11 DOI: 10.17504/protocols.io.e6nvwkyedvmk/v1
Abstract
A recipe to make lithium acetate / borate agarose gel electrophoresis buffer. This buffer has a lower ionic strength than TAE, but maintains its ability to resolve both small and large bands.
Additionally, it is MUCH less expensive, and if you are short on time (like in a lab course!) you can run the gel at a higher voltage.
From https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0011318 via https://bitesizebio.com/25078/faster-even-cooler-dna-gels/
Steps
25X Buffer Concentrate
1. Make sure it's the dihydrate, not anhydrous lithium acetate! If you're using anhydrous, weigh out 16.5g instead.
Weigh out 25.5g
Note
2.
Weigh out 15.5g
3.
Mix the lithium acetate and boric acid with deionized water to a final volume of 1L
4.
Pipette a small amount onto a
1X Buffer
5.
Dilute 40mL of 25X stock solution with 960mL deionized H2O. Mix well.
