In vivo fluorescent labeling and tracking of extracellular matrix

Adrian Fischer, Donovan Correa-Gallegos, Juliane Wannemacher, Simon Christ, Hans-Günther Machens, Yuval Rinkevich

Published: 2023-08-08 DOI: 10.1038/s41596-023-00867-y

fluorescent labeling

extracellular matrix

N-hydroxysuccinimide esters

tissue dynamics

organ development

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Genetic manipulation and immortalized culture of ex vivo primary human germinal center B cells

The cell-line-derived subcutaneous tumor model in preclinical cancer research

Subcellular patch-clamp techniques for single-bouton stimulation and simultaneous pre- and postsynaptic recording at cortical synapses

NovoSpaRc: flexible spatial reconstruction of single-cell gene expression with optimal transport

Genome-wide pooled CRISPR screening in neurospheres

High-throughput cultivation and identification of bacteria from the plant root microbiota

Viral crosslinking and solid-phase purification enables discovery of ribonucleoprotein complexes on incoming RNA virus genomes

Mitochondrial single-cell ATAC-seq for high-throughput multi-omic detection of mitochondrial genotypes and chromatin accessibility

Addendum: Micro-CT acquisition and image processing to track and characterize pulmonary nodules in mice

Multiplexed single-cell proteomics using SCoPE2

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References

Baron, C. S. & van Oudenaarden, A. Unravelling cellular relationships during development and regeneration using genetic lineage tracing. Nat. Rev. Mol. Cell Biol. 20, 753–765 (2019).
Kashimoto, R. et al. Lattice-patterned collagen fibers and their dynamics in axolotl skin regeneration. iScience 25, 104524 (2022).
Hastings, J. F., Skhinas, J. N., Fey, D., Croucher, D. R. & Cox, T. R. The extracellular matrix as a key regulator of intracellular signalling networks. Br. J. Pharmacol. 176, 82–92 (2019).
Manou, D. et al. The complex interplay between extracellular matrix and cells in tissues. Methods Mol. Biol. 1952, 1–20 (2019).
Loganathan, R. et al. Extracellular matrix motion and early morphogenesis. Development 143, 2056–2065 (2016).
Sivakumar, P. et al. New insights into extracellular matrix assembly and reorganization from dynamic imaging of extracellular matrix proteins in living osteoblasts. J. Cell Sci. 119, 1350–1360 (2006).
Aufschnaiter, R. et al. In vivo imaging of basement membrane movement: ECM patterning shapes Hydra polyps. J. Cell Sci. 124, 4027–4038 (2011).
Zamir, E. A., Czirók, A., Cui, C., Little, C. D. & Rongish, B. J. Mesodermal cell displacements during avian gastrulation are due to both individual cell-autonomous and convective tissue movements. Proc. Natl Acad. Sci. USA 103, 19806–19811 (2006).
Czirók, A., Rongish, B. J. & Little, C. D. Extracellular matrix dynamics during vertebrate axis formation. Dev. Biol. 268, 111–122 (2004).
Dzamba, B. J. & DeSimone, D. W. Extracellular matrix (ECM) and the sculpting of embryonic tissues. Curr. Top. Dev. Biol. 130, 245–274 (2018).
Duffield, M. Dynamic views. In Practical App Development with Aurelia Ch. 18, 185–194 (Apress, 2018).
Pankov, R. & Yamada, K. M. Non‐radioactive quantification of fibronectin matrix assembly. Curr. Protoc. Cell Biol. 25, 1–9 (2004).
Aper, S. J. A. et al. Colorful protein-based fluorescent probes for collagen imaging. PLoS ONE 9, 1–21 (2014).
Leonard, A. K. et al. Methods for the visualization and analysis of extracellular matrix protein structure and degradation. Methods Cell Biol. 143, 79–95 (2019).
Arnoldini, S. et al. Novel peptide probes to assess the tensional state of fibronectin fibers in cancer. Nat. Commun. 8, 1793 (2017).
Keeley, D. P. et al. Comprehensive endogenous tagging of basement membrane components reveals dynamic movement within the matrix scaffolding. Dev. Cell 54, 60–74 (2021).
Morris, J. L. et al. Live imaging of collagen deposition during skin development and repair in a collagen I–GFP fusion transgenic zebrafish line. Dev. Biol. 441, 4–11 (2018).
Ohashi, T., Kiehart, D. P. & Erickson, H. P. Dual labeling of the fibronectin matrix and actin cytoskeleton with green fluorescent protein variants. J. Cell Sci. 115, 1221–1229 (2002).
Lu, Y. et al. Live imaging of type I collagen assembly dynamics in osteoblasts stably expressing GFP and mCherry-tagged collagen constructs. J. Bone Miner. Res. 33, 1166–1182 (2018).
Poole, J. J. A. & Mostaço-guidolin, L. B. Optical microscopy and the extracellular matrix structure: a review. Cells 10, 1760 (2021).
Berg, E. A. & Fishman, J. B. Labeling antibodies. Cold Spring Harb. Protoc. 2020, 252–263 (2020).
Fischer, A. et al. Neutrophils direct preexisting matrix to initiate repair in damaged tissues. Nat. Immunol. 23, 518–531 (2022).
Correa-Gallegos, D. et al. Patch repair of deep wounds by mobilized fascia. Nature 576, 287–292 (2019).
Wan, L. et al. Connexin43 gap junction drives fascia mobilization and repair of deep skin wounds. Matrix Biol. 97, 58–71 (2021).

In vivo fluorescent labeling and tracking of extracellular matrix

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