How to Improve the Reliability of qPCR Detection with Target Gene-optimized Internal Standards

Achieving robust qPCR results requires both sensitive and accurate detection of target genes. This is particularly challenging for pathogen detection in field samples because the absence of a detection signal does not necessarily indicate the absence of the pathogen. Inhibitors present in the sample can suppress the pathogen signal, leading to false-negative results. The presented protocol details the use of an optimized internal standard for detecting CLas in HLB-infected citrus samples.

05_target-gene-optimized_Internal_standard_20240723.pdf