Global analysis of RNA-binding protein dynamics by comparative and enhanced RNA interactome capture

Joel I. Perez-Perri, Marko Noerenberg, Wael Kamel, Caroline E. Lenz, Shabaz Mohammed, Matthias W. Hentze, Alfredo Castello

Published: 2020-11-17 DOI: 10.1038/s41596-020-00404-1

Proteomics

RNA-binding proteins

RNA interactome capture

Locked Nucleic Acid

SILAC and TMT

AI 解读

Genetic manipulation and immortalized culture of ex vivo primary human germinal center B cells

The cell-line-derived subcutaneous tumor model in preclinical cancer research

Subcellular patch-clamp techniques for single-bouton stimulation and simultaneous pre- and postsynaptic recording at cortical synapses

NovoSpaRc: flexible spatial reconstruction of single-cell gene expression with optimal transport

Genome-wide pooled CRISPR screening in neurospheres

High-throughput cultivation and identification of bacteria from the plant root microbiota

Viral crosslinking and solid-phase purification enables discovery of ribonucleoprotein complexes on incoming RNA virus genomes

Mitochondrial single-cell ATAC-seq for high-throughput multi-omic detection of mitochondrial genotypes and chromatin accessibility

Addendum: Micro-CT acquisition and image processing to track and characterize pulmonary nodules in mice

Multiplexed single-cell proteomics using SCoPE2

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Interactions between RNA-binding proteins (RBPs) and RNAs are critical to cell biology. However, methods to comprehensively and quantitatively assess these interactions within cells were lacking. RNA interactome capture (RIC) uses invivo UV crosslinking,
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
References
ACKNOWLEDGMENTS
ACKNOWLEDGMENTS

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Global analysis of RNA-binding protein dynamics by comparative and enhanced RNA interactome capture

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